ABSTRACT
Objective To investigate the effect of silencing MFG-E8 gene on the sensitivity of SKOV3 cells to anticancer drugs and related mechanisms. Methods SKOV3 cells were transfected with MFG-E8 siRNA (Msi) and NC siRNA (Csi), respectively and the efficiency of transfection was confirmed by Western blot. The sensitivity of SKOV3 cells to cisplatin was observed by CCK-8 assay after transfection. The mRNA expression of ABCB1 and ABCC1 were detected by qRT-PCR. Effect of silencing MFG-E8 on the expression of ETM-related protein was detected by qRT-PCR and Western blot. Results MFG-E8 siRNA could effectively silence the expression of MFG-E8 protein. With the increasing drug concentration, the proliferation inhibition rate of each group also increased, and the cell proliferation inhibition rate of MFG-E8 siRNA group increased significantly (P < 0.01). Compared with NC siRNA group, downregulation of MFG-E8 expression led to decreased SKOV3 cell proliferation at 48h or 72h after 3 μg/ml cisplatin treat ment (P < 0.05). qRT-PCR results showed that the mRNA expression of ABCB1 and ABCC1 in Msi group were significantly lower than those in Csi group. qRT-PCR and Western blot results showed that silencing MFG-E8 gene down-regulated the mRNA and protein expression of N-Cadherin, Vimentin and Snail and up-regulated the expression of E-Cadherin. Conclusion Silencing the MFG-E8 gene can increase the sensitivity of SKOV3 cells against anti-tumor drugs and down-regulate the mRNA expression of ABCB1 and ABCC1, which may be related to the inhibition of EMT progression.
ABSTRACT
Objective To observe the effects of berberine ( BBR) combined with ethinylestradiol cyproterone acetate in the treatment of obese women with polycystic ovary syndrome ( PCOS) and the influence on vascular endo-thelial function.Methods 50 patients with PCOS were randomly divided into two groups.The observation group was given ethinylestradiol cyproterone acetate 1 piece of qn, BBR 300 mg tid.The control group was given placebo to replace BBR,the other treatment was the same as the observation group.The patients were treated for three menstrual cycles.The two groups were regulated the way of life.Before and after treatment,the body mass index (BMI),waist hip ratio (WHR),follicle-stimulating hormone (FSH),luteinizing hormone (LH),testosterone (T),fasting blood glucose ( FPG ) , fat cholesterol ( TC ) , triglycerides ( TG ) , insulin resistance index ( HOMA -IR ) , select E (E-selection),von willebrand factor (vWF) were tested.Results After treatment,these indicators of fat,sugar, insulin resistance,lipid metabolism disorders of sex hormone,vascular endothelial function in the observation group were more improved than the control group [(23.01 ±1.37),(0.81 ±0.03),(7.85 ±0.98),(4.12 ±0.55), (1.87 ±0.23),(2.56 ±0.67),(7.80 ±2.14),(1.46 ±0.78),(37.13 ±9.02),(142.15 ±43.26)vs (23.57 ± 1.40),(0.86 ±0.04),(87.22 ±11.38),(4.88 ±0.58),(2.25 ±0.24),(4.55 ±0.97),(9.96 ±3.20),(1.67 ±0.80), (24.36 ±7.48),(116.27 ±33.56)],there were statistically significant differences(t=3.85,3.92,4.28,4.32, 4.43,9.14,9.06,6.37,11.35,14.58,all P<0.05).Conclusion BBR combined with ethinylestradiol cyproterone acetate in the treatment of PCOS is not only effectively improved many pathophysiological changes,such as IR,high androgen levels,also protected vascular endothelial function,thereby reducing the risk of cardiovascular disease.